β-(1,3)-D-glucan from <em>Pleurotus ostreatus</em> correlates with lower plasma IL-6, IL-1β, HOMA-IR, and higher pancreatic beta cell count in High-Fat and High-Fructose Diet (HFFD) rats

Introduction: The increasing consumption of high-fat and high-fructose foods contributes to the prevalence global obesity. Low-grade chronic inflammation in obesity is a significant risk factor for insulin resistance type 2 diabetes. Therefore, this study aimed determine effect β-(1,3)-D-glucan from oyster mushroom (Pleurotus ostreatus) extract on rats fed with diet. Design Methods: This experimental was conducted 35 male Sprague-Dawley aged eight weeks. were divided into groups given normal (N) diet, diet (HFFD), D1 (HFFD+125 mg/kg BW β-glucan), D2 (HFFD+250 β glucan), D3 (HFFD+375 β-glucan) an intervention 14 IL-6 IL-1β levels measured by ELISA method, while HOMA-IR (Homeostatic Model Assessment Insulin Resistance) calculated fasting (ng/mL) x blood glucose (mg/dL)/405 formula. Pancreatic beta-cell counts hematoxylin eosin (H&E) staining. Results: results showed no differences between treatment groups. However, there pancreatic There negative correlations dose β-glucan IL-6, IL-1β, levels. Also, positive correlation number beta cells. Conclusions: Administration prevented hyperglycemia resistance, also reduced HFFD regardless weight gain.